He graduated from Karadeniz Technical University, Faculty of Engineering, Department of Mechanical Engineering. He completed his master's degree in Bahçeşehir University Urban Systems and Transportation Management Programme.

In 1995, he started to work at the Directorate of Transport Coordination in Istanbul Metropolitan Municipality. He worked as an engineer and manager at IETT. He worked as Deputy Traffic Manager and Directorate of Transport Coordination, Head of Transport Department and Deputy Secretary General for Transport, Environment and Technology Investments at Istanbul Metropolitan Municipality. During these positions, he ensured that Istanbul Transport and Logistics Master Plan studies were carried out and implemented. Within the framework of these studies, roads, junctions and tunnels were planned for the development of Istanbul Highway infrastructure.

In 2019, he was appointed as Deputy Minister of Transport and Infrastructure. While serving as Deputy Minister of the Ministry of Transport and Infrastructure, he was appointed as the Minister of Transport and Infrastructure on 28 March 2020. During his tenure as the Minister of Transport and Infrastructure, he has accomplished several major projects in the fields of communication, transport and infrastructure in order to ensure Türkiye's national and international integration. By creating the 2053 Transport and Logistics Master Plan, he determined Türkiye's investments to be implemented until 2053.

Karaismailoğlu is married and has 2 children.